Sequences from confirmation amplicon


Confirmation sequences for line 282H01 and hit At5g42540 are shown.
Line ID 282H01
Locus At5g42540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17011774
Primers used for PCR 10mi - 8474, show primer details
   
Sequence Name 35-K114086-0022-282-H01C-10mi
GenBank Accession LN501914 [GenBank]
Sequencing primer gene specific primer, 10mi: 5'-AGCCTCAAAGCAGAAGTAAGTCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K114086-0022-282-H01C-10mi
ATTTACAATTTTAGCTCTTCCGCCTTTTTTTGAACTGCATTTCTTTTGTTTAATTTTATC
ATGTGCTGGTTTAAGTGTTAACTACTCCATTTTAAATTTTTGGTCTTGAACTTTTCTTTC
TTATTGTAAGTGCACTAAAGTACACAGCGNGTTTTCAGGAACTAGATAACGAAGAAGACT
TAAAGTTTAAGCTTAAGAAACTACTGCGGGATAAAGGTGATGGGTTCAGGTCAGGAAATG
GAGAACAAGATAAGGTCATATTTCTATTTTTTCAGCTGTGTGTTCATCAATGATTTCATG
AGTGTGTAATCTTCATAAGTTTGTTTGATTTCTGTTTAATCAATCATTTAATCACGCTGG
CAAGCTCACATGGCTGTTGTATTATCAGGTTAAGCTAAATAAAGTAGGATGGAGAGAGAG
ATATTACGAAGAGAAATTCGCAGCTAAGTCTGTGGAGGAAATGGAACAAATACGCAGAGA
TGTTGTGAGTTGCTCATATGTTTAATTTGATGAAAATAAATTACATTTAAGTCACCCATA
ACGTATCAATTTACTGATCTTATCAATTATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 59-K114085-0022-282-H01C-8409
GenBank Accession LN510059 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K114085-0022-282-H01C-8409
TTACAATTGAATAAATTGATAAGATCAGTAAATTGATACGTTATGGGTGACTTAAATGTA
ATTTATTTTCATCAAATTAAACATATGAGCAACTCACAACATCTCTGCGTATTTGTTCCA
TTTCCTCCACAGACTTAGCTGCGAATTTCTCTTCGTAATATCTCTCTCTCCATCCTACTT
TATTTAGCTTAACCTGATAATACAACAGCCATGTGAGCTTGCCAGCGTGATTAAATGATT
GATTAAACAGAAATCAAACAAACTTATGAAGATTACACACTCATGAAATCATTGATGAAC
ACACAGCTGAAAAAATAGAAATATGACCTTATCTTGTTCTCCATTTCCTGACCTGAACCC
ATCACCTTTATCCCGCAGTAGTTTCTTAAGCTTAAACTTTAAGTCTTCTTCGTTATCTAG
TTCCTGAAAACCGCTGTGTACTTTAGTGCACTTACAATAAGAAAGAAAAGTTCAAGACCA
AAAATTTAAAATGGAGTAGTTAACACTTAAACCAGCACATGATAAAATTAAACAAAAGAA
ATGCAGTTCAAAAAAAGGCGAGAAGAGCTAAAAATTTGTTAAAATTCCTCCTCGGATACG
AGATGACTTACTTCTGNTTTGAGGC

Last Updated on 06.12.2018 16:11