Genotyping and primer details


Genotyping and primer details for 282H01 - At5g42540
Line ID 282H01
Locus At5g42540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:17011774 of line 282H01 Chr5:17011774
   
Insertion allele amplicon 8474 - 10mi
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 10mi: 5'-AGCCTCAAAGCAGAAGTAAGTCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:17011169
   
wt amplicon (over InsPos) 02mo - 10mi
wt amplicon start Chr5:17011169
wt amplicon end Chr5:17012245
wt amplicon size 1077 bp
Locus specific primer 10mi: 5'-AGCCTCAAAGCAGAAGTAAGTCAT-3'
2nd locus specific primer 02mo: 5'-CCTATTATTATGGCCATCTTGTCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11