Sequences from confirmation amplicon


Confirmation sequences for line 283F10 and hit At4g12120 are shown.
Line ID 283F10
Locus At4g12120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7260855
Primers used for PCR ST11 - 8474, show primer details
   
Sequence Name 17-K103492-0022-283-F10C-ST11
GenBank Accession LN493816 [GenBank]
Sequencing primer gene specific primer, ST11: 5'-AAGAAATGCCTTCTTGTGTGATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K103492-0022-283-F10C-ST11
GAACTTGTCCATTACAGTACCTAAATAATACATTCATATGTTCAGTGAATCAATGAGCAA
ACCCATAGAATCAAAAACAATAAAGTAAAACCAAGATTTTCAAGGTAAATTTATAAGAAA
GCTGGATCAAAAAAAATTACCTTCCAAGTTAATTTAGAGCTTCCATTTCTTTCTGGCCTA
AGCATTTCACAAAGTAATCCTGAAACCAAAATGGACATAGTAATTACCTAAAATGCAAAA
CCCTCATTTTCCTGATATGCAAAGTTTTTGCTTCAATGAACATTGTGATCACGATGACCT
AATTCGAAACTCCATAAACCCAATGATTTTCTCATACTAATCCAACGAAACCCTAGAACC
TAGAGACCAGAAGCTTCCAGACAAACCCTATAACAGAGTCTAATGATCTAATCAAAAACC
CAATTTCGAAAAATCCAAAATTGGTAAGAGAAAGGGTAAAAGAAGCTAACTTTCTCTGGT
GATTTGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 41-K103493-0022-283-F10C-8409
GenBank Accession LN504049 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K103493-0022-283-F10C-8409
TTACATTGAATAATCCTGACAATCACCAGAGAAAGTTAGCTTCTTTTACCCTTTCTCTTA
CCAATTTTGGATTTTTCGAAATTGGGTTTTTGATTAGATCATTAGACTCTGTTATAGGGT
TTGTCTGGAAGCTTCTGGTCTCTAGGTTCTAGGGTTTCGTTGGATTAGTATGAGAAAATC
ATTGGGTTTATGGAGTTTCGAATTAGGTCATCGTGATCACAATGTTCATTGAAGCAAAAA
CTTTGCATATCAGGAAAATGAGGGTTTTGCATTTTAGGTAATTACTATGTCCATTTTGGT
TTCAGGATTACTTTGTGAAATGCTTAGGCCAGAAAGAAATGGAAGCTCTAAATTAACTTG
GAAGGTAATTTTTTTTGATCCAGCTTTCTTATAAATTTACCTTGAAAATCTTGGTTTTAC
TTTATTGTTTTTGATTCTATGGGTTTGCTCATTGATTCACTGAACATATGAATGTATTAT
TTAGGTACTTGTAATGGACAAGTTCACTGTGAAGATAATGTCATCTGCTTGCAAAATGTC
TGAGATCACACAAGAAGGCATTTC

Last Updated on 06.12.2018 16:11