Genotyping and primer details


Genotyping and primer details for 283F10 - At4g12120
Line ID 283F10
Locus At4g12120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:7260855 of line 283F10 Chr4:7260855
   
Insertion allele amplicon 8474 - ST11
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer ST11: 5'-AAGAAATGCCTTCTTGTGTGATCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:7260310
   
wt amplicon (over InsPos) 12cu - ST11
wt amplicon start Chr4:7260310
wt amplicon end Chr4:7261352
wt amplicon size 1043 bp
Locus specific primer ST11: 5'-AAGAAATGCCTTCTTGTGTGATCT-3'
2nd locus specific primer 12cu: 5'-AGTGAATCATAGTTTGACTTGGGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11