Sequences from confirmation amplicon


Confirmation sequences for line 286G04 and hit At1g30330 are shown.
Line ID 286G04
Locus At1g30330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10689539
Primers used for PCR KS40 - 8474, show primer details
   
Sequence Name 38-K032982-022-286-G04C-KS40
GenBank Accession LN502889 [GenBank]
Sequencing primer gene specific primer, KS40: 5'-ATTTGGCTGATGATTTATTTCAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K032982-022-286-G04C-KS40
GTTTTTTTAGGGTTTTAGATTTGGTTGGTGAATGGGTGGGAGGTGGAGGGAAACAGTTAA
AAAAGTTATGCTTTTAGTGTCTCTTCTTCATAATTACATTTGGGCATCTTGAAATCTTTG
GATCTTTGAAGAAACAAAGTTGTGTTTTTTTTTTTGTTCTTTGTTGTTTGCTTTTTAAGT
TAAAAAAAAAAATGAAATTATCTTCAGCGGGGTTTAATCCTCAACCTCATGAAGGTAATT
CTCTCTTTTCTCTCTCTATGGCTTTGATACTTCTAGCAAATAGTGATTTTAAACTAACTT
TGTGAGTTTTGGATTGAAAGTTACAGGAAAAAAAAAAGTTCTTAATTCTAAGCTCTGGCA
GGCTTGTGCTGGTCCTCTTGTCTCACTACCCCCGGTTGGAAGCAAATTTGTGTATTTTCC
TCAAGGTCACAGTGAACAGGTTTTTGTCCCCTTCCTTTTAGGATTTGGATTCAAATCTTT
CCTTTTTGGTTACAGGTTACTTGAAAATGTTTTGGGGTTTTCAGGTTGCTGCTTCAACCA
ACAAAAAAGTAAATGCTCATATACCAAATTATCCAAGCTTGCATCCGCAGCTTATCTGTC
AGCTTCATAATGTTACAATGCAGGTAAGGAACTCACTATCATTTTTTATGACAAACTTTT
CAAAGTGTCTTTTCGGTTTGGGGGTTAGAAGGAGGGATCACCAATGATTATGATGTCCGG
GAAATGGCTTCATGTCCGGGAAATCTACTTGGATCACCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCATTTTTTTTTCAATTCAAAAATGTAAATGTCCGCGGC
GTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCG
   
Sequence Name 14-K032982-022-286-G04C-8409
GenBank Accession LN492403 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K032982-022-286-G04C-8409
ATTTCCCGGACATCATACTCATTGCTGATCCATCATACTAACACACAAACAGAAAAGACA
CTTAGAAAAGTTTGTCATAAAAACTGATAGTGAGTTCCTTACATGCATTGTAACATTATG
AAGCTGACAGATAAGCTGCGGATGCAAGCTCGGATAATTTGGTATATGAGCATCTACTTC
TTTGTTGGTCGAAGCAGCAACCTGAAAACCACAAAACATTCTCAAGTAACCTGTAACCAA
AAAGGAAAGATTTGAATCCAAATCATAAAAGGAAGGAGACAAAAACCTGTTCACTGTGAC
CTTGAGGAAAATACACAACTCTGCTTCCAACAGGAGGTAGTGAGACAAGAGGACCAGCAC
AAGCATGCCAGAGCTCAGAATTAAGAACTCTTTTCTCTCCTGTAACTTTCAATCCAAAAC
TCACAAAGTTAGTTTAAAATCACTATTTGCTAGAAGTATCAAAGCAATAGAGAGAGAAAA
GAGAGAATTACCTTCATGAGGTTGAGGATTAAACCCAGCTGAAGATAATCTCATTTTTTA
TTCTAACTTAAAAAGCAAACAACAAAGAACAAAAAAAAAAACCCAACTTTGTTTCTTCAA
AGATCCAAAGATTTCAAGATGCCCAAATGTAATTATGAAAAAAAGACACTAAAAGCATAA
CTTTTTTAACTGTTTCCCTCCACCTCCCACCCATTCACCAACCAAATCTAAAAACCCTAA
AAAAAACAAATTTATTTCTTCTTCCCTGAAAT

Last Updated on 06.12.2018 16:11