Genotyping and primer details


Genotyping and primer details for 286G04 - At1g30330
Line ID 286G04
Locus At1g30330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:10689539 of line 286G04 Chr1:10689539
   
Insertion allele amplicon 8474 - KS40
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KS40: 5'-ATTTGGCTGATGATTTATTTCAGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:10690270
   
wt amplicon (over InsPos) 13sl - KS40
wt amplicon start Chr1:10689201
wt amplicon end Chr1:10690270
wt amplicon size 1070 bp
Locus specific primer KS40: 5'-ATTTGGCTGATGATTTATTTCAGG-3'
2nd locus specific primer 13sl: 5'-ATAAGATGAATTACCAAGGGAGGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11