Sequences from confirmation amplicon


Confirmation sequences for line 297A03 and hit At5g12210 are shown.
Line ID 297A03
Locus At5g12210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3949501
Primers used for PCR 33sm - 8474, show primer details
   
Sequence Name 23-K116610-0022-297-A03C-33sm
GenBank Accession LN496713 [GenBank]
Sequencing primer gene specific primer, 33sm: 5'-TTTACAGAATTAACAAGGCCAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K116610-0022-297-A03C-33sm
TCATGACTAGATACTCAGATGTTTATTTTTCTGTAGTCGACGTGCTATTGATACATCTTT
ATGTAAATTGTATAGGACAGTTTCATGGTTGGGAGATATGGTAAAAAGACGGTATTCTTT
ATTTGTTTCTTCTGTTACTGCAGTTTTCTGCTGCGTTGGTGCTCTTGCTATCACTGGGAG
TCTCCATCATGTTGATAAGGACTCACTTGGTTGGTGGTTATGTGAAAGACAATTAAAGGC
TGGGGGCTTAAATGGACGTCCTGAGAAACTCGCTGATGTAGGCATTAAGCTCATCTCCAA
TGAGAGAGACCTTTGTATGATTTCCTTGGTTCTTACACATTTTTTACTGTTCTTATTTCC
AGGTTTGCTATTCTTGGTGGGTCCTATCGAGCCTAATCATGATAGATAGAGTTCACTGGA
TCGACAAAGCAAAGCTTGTCAAGTTCATTCTGGACTGTCAGGTTCGTCTCCAACAAGAAA
ATTCATATCGTTACTAATAAGCCAGTTTTGATTGAAAGCTCTCATCTCATCTATCTAAAT
TTGAATTTGAATAGGATTTGGACAACGGAGGCATCTCAGACCGACCCGAGGATGCTGTTG
ATATCTTCCACACCTACTTTGGAGTTGCGGGTAACTTTCATTTGTCTCTTCTTTTAGCTC
ATCTCTTGCTGTGAAGCCAAAAGTGGTCTTATTTCATCAAATTGGTGCAGGATATATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CA
   
Sequence Name 07-K116609-0022-297-A03C-8409
GenBank Accession LN489438 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K116609-0022-297-A03C-8409
ATTTCATTGAATATATCCTGCACAATTTGATGAAATAAGACCACTTTTGGCTTCACAGCA
AGAGATGAGCTAAAAGAAGAGACAAATGAAAGTTACCCGCAACTCCAAAGTAGGTGTGGA
AGATATCAACAGCATCCTCGGGTCGGTCTGAGATGCCTCCGTTGTCCAAATCCTATTCAA
ATTCAAATTTAGATAGATGAGATGAGAGCTTTCAATCAAAACTGGCTTATTAGTAACGAT
ATGAATTTTCTTGTTGGAGACGAACCTGACAGTCCAGAATGAACTTGACAAGCTTTGCTT
TGTCGATCCAGTGAACTCTATCTATCATGATTAGGCTCGATAGGACCCACCAAGAATAGC
AAACCTGGAAATAAGAACAGTAAAAAATGTGTAAGAACCAAGGAAATCATACAAAGGTCT
CTCTCATTGGAGATGAGCTTAATGCCTACATCAGCGAGTTTCTCAGGACGTCCATTTAAG
CCCCCAGCCTTTAATTGTCTTTCACATAACCACCAACCAAGTGAGTCCTTATCAACATGA
TGGAGACTCCCAGTGATAGCAAGAGCACCAACGCAGCAGAAAACTGCAGTAACAGAAGAA
ACAAATAAAGAATACCGTCTTTTTACCATATCTCCCAACCATGAAACTGTCCTATACAAT
TTACATAAAGATGTATCAATAGCACGTCGACTACAGAAAAATAAACATCTGAGTATCTAG
TCATTGGAATAGTTAAACGGCAGTGATGCGGTTGGCCTTGTT

Last Updated on 06.12.2018 16:11