Genotyping and primer details


Genotyping and primer details for 297A03 - At5g12210
Line ID 297A03
Locus At5g12210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:3949501 of line 297A03 Chr5:3949501
   
Insertion allele amplicon 8474 - 33sm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 33sm: 5'-TTTACAGAATTAACAAGGCCAACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:3948744
   
wt amplicon (over InsPos) 03ur - 33sm
wt amplicon start Chr5:3948744
wt amplicon end Chr5:3949806
wt amplicon size 1063 bp
Locus specific primer 33sm: 5'-TTTACAGAATTAACAAGGCCAACC-3'
2nd locus specific primer 03ur: 5'-CAACTTCGTTTCCATTACATCAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11