Sequences from confirmation amplicon


Confirmation sequences for line 310C11 and hit At1g05990 are shown.
Line ID 310C11
Locus At1g05990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1818983
Primers used for PCR BV15 - 8474, show primer details
   
Sequence Name 16-K105564-0022-310-C11C-BV15
GenBank Accession LN493430 [GenBank]
Sequencing primer gene specific primer, BV15: 5'-AGATTTTTGAATTCCTGCCCTTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K105564-0022-310-C11C-BV15
AAGGTAAGATTTTCTCGGCCTCTGGAGCCCTCACCATTATCTTTTCTTCCTCCACACGCA
ACACTACAACTTTAAAAGAGATGGATCCGACAGAGCTAAAACGCGTGTTCCAAATGTTCG
ACAAGAACGGGGACGGGACGATCACGGGGAAAGAGCTGAGCGAGACACTACGAAGCCTAG
GGATTTACATCCCGGACAAGGAGTTGACTCAAATGATCGAGAAGATCGACGTGAATGGTG
ATGGGTGCGTGGACATAGACGAGTTTGGTGAGCTATACAAGACGATAATGGACGAGGAAG
ACGAAGAAGAAGAGGACATGAAAGAAGCTTTCAATGTGTTTGATCAGAATGGAGATGGGT
TTATAACGGTGGATGAATTGAAGGCGGTTTTGTCTTCCTTGGGGCTCAAACAAGGTAAGA
CCTTGGACGATTGTAAGAAGATGATAAAGAAAGTGGATGTTGATGGTGATGGTAGGATAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCC
   
Sequence Name 64-K105565w-0022-310-C11C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K105565w-0022-310-C11C-8409
ATTTACATTGAATATATCCTACCATCACCATCAACATCCACTTTCTTTATCATCTTCTTA
CAATCGTCCAAGGTCTTACCTTGTTTGAGCCCCAAGGAAGACAAAACCGCCTTCAATTCA
TCCACCGTTATAAACCCATCTCCATTCTGATCAAACACATTGAAAGCTTCTTTCATGTCC
TCTTCTTCTTCGTCTTCCTCGTCCATTATCGTCTTGTATAGCTCACCAAACTCGTCTATG
TCCACGCACCCATCACCATTCACGTCGATCTTCTCGATCATTTGAGTCAACTCCTTGTCC
GGGATGTAAATCCCTAGGCTTCGTAGTGTCTCGCTCAGCTCTTTCCCCGTGATCGTCCCG
TCCCCGTTCTTGTCGAACATTTGGAACACGCGTTTTAGCTCTGTCGGATCCATCTCTTTT
AAAGTTGTAGTGTTGCGTGTGGAGGAAGAAAAGATAATGGTGAGGGCTCCACGAGGCCGA
GAAAATCTTTTACCCTTTCCTGGCTTCTTGGATAAGGGCAG

Last Updated on 06.12.2018 16:11