Genotyping and primer details


Genotyping and primer details for 310C11 - At1g05990
Line ID 310C11
Locus At1g05990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:1818983 of line 310C11 Chr1:1818983
   
Insertion allele amplicon 8474 - BV15
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BV15: 5'-AGATTTTTGAATTCCTGCCCTTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1818466
   
wt amplicon (over InsPos) AR16 - BV15
wt amplicon start Chr1:1818466
wt amplicon end Chr1:1819485
wt amplicon size 1020 bp
Locus specific primer BV15: 5'-AGATTTTTGAATTCCTGCCCTTAT-3'
2nd locus specific primer AR16: 5'-CAATATGCGCTTGACAAAATTAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11