Sequences from confirmation amplicon


Confirmation sequences for line 315E04 and hit At1g75510 are shown.
Line ID 315E04
Locus At1g75510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28348234
Primers used for PCR HM20 - 8474, show primer details
   
Sequence Name 39-K029126-022-315-E04C-8409
GenBank Accession LN503239 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K029126-022-315-E04C-8409
GATCCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGGTATACTTATTACCTTTGT
TTTGGTATTAATCAGTTCAAAATGTATATGGTTGGTGCTGAGTATGGGAACATGCCAAAG
TGCTACGCTTTGAACATGTTCACAGACTTTGTTCCGATGGGGGGCTTTTCCGATGTTAAT
CAAGGTTTGGATTTTACTTTGTTCTGATTGAATACATTAGATTTTTGTTTCTTCGTTTGC
TTTTATTCTCATACTTGGCAAGCTACTCTAGGTTGTGCAGCTGCTGAAGGAAAGGTTGAT
CACAAATTCGATATGAAGCCATATGGTGAAACCATTGAAGAATATGCTAGGCTTTGTCGT
GAGAGAACGAGCAAAGCCATGGTTAAAAATCGACAGATTCAGGTCTGTTTTATATTTTCA
CATTCAGGTGTTTGTTTTCCTTTGCTTCTCCTATACTGGCTCTTAAGTGTAAGGACTTTT
CTTCCTTGAATTTGCGCTAAAGGTTATTGATAATGACCGCGGAGTTCATATGAGGCCCAT
GCCTGGAATGCTTGGTTTAGTTTCCTCCAATTCCAAGGTAAAAATGTTATTCTCTTCTCA
ATGATTTTGCATATATGGTGTAATCATTGAAATGGTAAAGAGTTAGTG
   
Sequence Name 40-K029126-022-315-E04C-HM20
GenBank Accession LN503592 [GenBank]
Sequencing primer gene specific primer, HM20: 5'-TTGAGTATTGAAGCTGGATTGTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K029126-022-315-E04C-HM20
GTTGCTTCACTAACTCTTTACCATTTCAATGATTACACCATATATGCAAAATCATTGAGA
AGAGAATAACATTTTTACCTTGGAATTGGAGGAAACTAAACCAAGCATTCCAGGCATGGG
CCTCATATGAACTCCGCGGTCATTATCAATAACCTTTAGCGCAAATTCAAGGAAGAAAAG
TCCTTACACTTAAGAGCCAGTATAGGAGAAGCAAAGGAAAACAAACACCTGAATGTGAAA
ATATAAAACAGACCTGAATCTGTCGATTTTTAACCATGGCTTTGCTCGTTCTCTCACGAC
AAAGCCTAGCATATTCTTCAATGGTTTCACCATATGGCTTCATATCGAATTTGTGATCAA
CCTTTCCTTCAGCAGCTGCACAACCTAGAGTAGCTTGCCAAGTATGAGAATAAAAGCAAA
CGAAGAAACAAAAATCTAATGTATTCAATCAGAACAAAGTAAAATCCAAACCTTGATTAA
CATCGGAAAAGCCCCCCATCGGAACAAAGTCTGTGAACATGTTCAAAGCGTAGCACTTTG
GCATGTTCCCATACTCAGCACCAACCATATACATTTTGAACTGATTAATACCAAAACAAA
GGTAATAAGTATACCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTAT

Last Updated on 06.12.2018 16:11