Genotyping and primer details


Genotyping and primer details for 315E04 - At1g75510
Line ID 315E04
Locus At1g75510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28348234 of line 315E04 Chr1:28348234
   
Insertion allele amplicon 8474 - HM20
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer HM20: 5'-TTGAGTATTGAAGCTGGATTGTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28347601
   
wt amplicon (over InsPos) 25rd - HM20
wt amplicon start Chr1:28347601
wt amplicon end Chr1:28348682
wt amplicon size 1082 bp
Locus specific primer HM20: 5'-TTGAGTATTGAAGCTGGATTGTTG-3'
2nd locus specific primer 25rd: 5'-AGAAACTGCGACAAGAGAAAGAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11