Sequences from confirmation amplicon


Confirmation sequences for line 322A09 and hit At5g17330 are shown.
Line ID 322A09
Locus At5g17330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5714698
Primers used for PCR 19kh - 8474, show primer details
   
Sequence Name 35-K113531-0022-322-A09C-19kh
GenBank Accession LN501902 [GenBank]
Sequencing primer gene specific primer, 19kh: 5'-AGGGAAATGAAACTTACTTCAGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K113531-0022-322-A09C-19kh
CGATAAGGGGAATGAACATTCAAATATCCAAATCAATGCAATTATGCATTATTAATAAAA
GTCTTCAAACATTTGAAGTAAATATATTTCGATTAGCAGATACCACTCGTCTTCTTCCTG
TCGGCGACAAACTTCTTCCAGCCAGTGATGATATCTCTCTGCTTGTCGATATCGCTCTTC
TTCACCGTGACCATCAAGTTATCGCTGTTAGATTCACTCTTCTCTTGTCCAAGTTATCGC
TCTTCTTCACCGTGACCATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 10-K113530-0022-322-A09C-8409
GenBank Accession LN490739 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K113530-0022-322-A09C-8409
ATCCTGATGGTCACGGTGAAGAAGAGCGATAACTTGGACAAGAGAAGAGTGAATCTAACA
GCGATAACTTGATGGTCACGGTGAAGAAGAGCGATATCGACAAGCAGAGAGATATCATCA
CTGGCTGGAAGAAGTTTGTCGCCGACAGGAAGAAGACGAGTGGTATCTGCTAATCGAAAT
ATATTTACTTCAAATGTTTGAAGACTTTTATTAATAATGCATAATTGCATTGATTTGGAT
ATTTGAATGTTTCATTCCCCTTTATCGCTGAACCAAGTTTCATTTCCCTTTATTGCTGAA
GTAAGTTTCATTTCC

Last Updated on 06.12.2018 16:11