Genotyping and primer details


Genotyping and primer details for 322A09 - At5g17330
Line ID 322A09
Locus At5g17330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:5714698 of line 322A09 Chr5:5714698
   
Insertion allele amplicon 8474 - 19kh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 19kh: 5'-AGGGAAATGAAACTTACTTCAGCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:5714983
   
wt amplicon (over InsPos) 10ky - 19kh
wt amplicon start Chr5:5714330
wt amplicon end Chr5:5714983
wt amplicon size 654 bp
Locus specific primer 19kh: 5'-AGGGAAATGAAACTTACTTCAGCA-3'
2nd locus specific primer 10ky: 5'-CTTGAAAGATAGCAGCTGTCACAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11