Sequences from confirmation amplicon


Confirmation sequences for line 339A12 and hit At2g45420 are shown.
Line ID 339A12
Locus At2g45420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18719991
Primers used for PCR 22kb - 8474, show primer details
   
Sequence Name 67-K113393-0022-339-A12C-8409
GenBank Accession LN511854 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K113393-0022-339-A12C-8409
CATTTCATTGATATGTATACAAAACAAATTCTAACTCGTAAAGTGTATACATAGGTGGTG
AATCTACAGGCAGAAGTTTCTTACTTACAAGCCCATTTGGCATCACTAGAGCTACCTCAA
CCGCAAACGCGGCCACAGCCTATGCCGCAACCGCAACCGCTCTTTTTCACTCCTCCTCCC
CCTCTTGCCATAACCGATTTACCTGCATCAGTCTCACCCTTGCCCTCAACCTATGACCTA
GCATCCATCTTCGATCAAACGACGTCTTCTTCGGCATGGGCCACGCAACAACGAAGATTC
ATTGATCCACGTCACCAATACGGTGTATCATCGTCTTCCTCTTCTGTCGCCGTGGGGCTC
GGTGGTGAAAACAGTCATGACCTCCAAGCATTAGCACATGAACTCCTCCATAGACAAGGA
TCTCCTCCCCCGGCAGCTACCGACCACTCGCCGTCTCGAACTATGTCTAGATGAGTTGGT
TTTGACGNATACAT
   
Sequence Name 86-K113394-0022-339-A12C-22kb
GenBank Accession LN514679 [GenBank]
Sequencing primer gene specific primer, 22kb: 5'-GATGTATCGATCAAAACCAACTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K113394-0022-339-A12C-22kb
CTGCCGGGGGAGGAGATCCTTGTCTATGGAGGAGTTCATGTGCTAATGCTTGGAGGTCAT
GACTGTTTTCACCACCGAGCCCCACGGCGACAGAAGAGGAAGACGATGATACACCGTATT
GGTGACGTGGATCAATGAATCTTCGTTGTTGCGTGGCCCATGCCGAAGAAGACGTCGTTT
GATCGAAGATGGATGCTAGGTCATAGGTTGAGGGCAAGGGTGAGACTGATGCAGGTAAAT
CGGTTATGGCAAGAGGGGGAGGAGGAGTGAAAAAGAGCGGTTGCGGTTGCGGCATAGGCT
GTGGCCGCGTTTGCGGTTGAGGTAGCTCTAGTGATGCCAAATGGGCTTGTAAGTAAGAAA
CTTCTGCCTGTAGATTCACCACCTATGTATACACTTTACGAGTTAGAATTTGTTTTGTAT
ACATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCACGGTTATTA

Last Updated on 06.12.2018 16:11