Genotyping and primer details


Genotyping and primer details for 339A12 - At2g45420
Line ID 339A12
Locus At2g45420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:18719991 of line 339A12 Chr2:18719991
   
Insertion allele amplicon 8474 - 22kb
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 22kb: 5'-GATGTATCGATCAAAACCAACTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:18720466
   
wt amplicon (over InsPos) 49ve - 22kb
wt amplicon start Chr2:18719508
wt amplicon end Chr2:18720466
wt amplicon size 959 bp
Locus specific primer 22kb: 5'-GATGTATCGATCAAAACCAACTCA-3'
2nd locus specific primer 49ve: 5'-ATCATTTTGTTTCTTCGCAATTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11