Sequences from confirmation amplicon


Confirmation sequences for line 340E01 and hit At1g51260 are shown.
Line ID 340E01
Locus At1g51260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19003592
Primers used for PCR 08ja - 8474, show primer details
   
Sequence Name 34-K112831-0022-340-E01C-08ja
GenBank Accession LN501478 [GenBank]
Sequencing primer gene specific primer, 08ja: 5'-TTTTGTGAAAACAATAGGCTCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K112831-0022-340-E01C-08ja
AGTCGAAGTTCAAAAATTAAATTTCATGGTTATTTGAAATAATAACAGTTTGGAAATATA
AAATTATACATAGATTTGACAATGAAATGTGGTTGCAATTGTTAGACAACAAGACTAAAT
GATCAAACTAGATTAACATTTATTACACATATTACACACTAACAAAAACAATTATGTTTA
CATTTGAGTGAAGGAACAATGCAGTGTAAAATATGTGGCTTAAATAGAGTTTTCTTTTGT
CCTAAGCAGAAATAAGCTGTTCTTGTAGAGGTCTCTTGGCTGGAGTTGAACGTTGTGACT
CAGACGATTGAATTAGTATTTGCATTGTTATAGTTGCAATGACCAAGAAGAACACAAACA
AAAGAATGATCTTCATGTATAACCAAATGAATGCAAATATGGAGAAAAAGAAAGAGTAAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCAGCGTTATTA
   
Sequence Name 05-K112829-0022-340-E01C-8409
GenBank Accession LN488432 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K112829-0022-340-E01C-8409
TTACATTGATATTACTCTTTCTTTTTCTCATATTTGCATTCATTTGGTTATACATGAAGA
TCATTCTTTTGTTTGTGTTCTTCTTGGTCATTGCAACTATAACAATGCAAATACTAATTC
AATCGTCTGAGTCACAACGTTCAACTCCAGCCAAGAGACCTCTACAAGAACAGCTTATTT
CTGCTTAGGACAAAAGAAAACTCTATTTAAGCCACATATTTTACACTGCATTGTTCCTTC
ACTCAAATGTAAACATAATTGTTTTTGTTAGTGTGTAATATGTGTAATAAATGTTAATCT
AGTTTGATCATTTAGTCTTGTTGTCTAACAATTGCAACCACATTTCATTGTCAAATCTAT
GTATAATTTTATGTTTCCAAACTGTTATTATTTCAAATAACCATGAAATTTAATTTTGTG
AACTTTCGACTCGTTGTCATTACATAGCCTTCTAACTTTCCTTTGAGCCTATTGTTTTCA
CAAA

Last Updated on 06.12.2018 16:11