Genotyping and primer details


Genotyping and primer details for 340E01 - At1g51260
Line ID 340E01
Locus At1g51260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:19003592 of line 340E01 Chr1:19003592
   
Insertion allele amplicon 8474 - 08ja
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 08ja: 5'-TTTTGTGAAAACAATAGGCTCAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:19003163
   
wt amplicon (over InsPos) 06ji - 08ja
wt amplicon start Chr1:19003163
wt amplicon end Chr1:19004163
wt amplicon size 1001 bp
Locus specific primer 08ja: 5'-TTTTGTGAAAACAATAGGCTCAAA-3'
2nd locus specific primer 06ji: 5'-TAACCGTTCATAACAATCAGCCTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11