Sequences from confirmation amplicon


Confirmation sequences for line 344C02 and hit At1g74710 are shown.
Line ID 344C02
Locus At1g74710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28071260
Primers used for PCR 13ve - 8474, show primer details
   
Sequence Name 71-K118051-0022-344-C02C-13ve
GenBank Accession LN512628 [GenBank]
Sequencing primer gene specific primer, 13ve: 5'-GTCATGAGAGAAAGGATCAAGGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K118051-0022-344-C02C-13ve
AGCACGCTAACAGAATACGATCAGAAGACACTGTTCCGTTTCCGTTTCCGTTTCCGTTCT
CGTTAGCTAGATCGAGAAGAAGATCGGGACGACCAACGTCACTGCGACGAGAGAAGAAAC
AGCGAGGCTGAATCTCATTCTGGGCTTGAAGCCAATCAATTGCTCCGATTTGCTGGTCAA
TTGGAACCTGCACCAAATCCAAATTAGGGTTTATTGCGCGGGAAAATTCGACTGTGATTT
GGGTTTTTTAAAAAACTCTGCGTTTTAATGAGAGTGACTGTATTTGATCGCCGCCTTGCC
TTTACAACAAATTGGATTCTAATCATTATTTTACTTATTTTCTGGCCCACAAAACACAAA
ATTATTACTTTTCTAGCTAAATTATCTTAGCATGGCCACACTAACTAAAAGTTAACAGTG
TTAAATTAGATTTCGAGGACTAAAAAAACATGATTATTCTTTCTAAAATGTATTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
   
Sequence Name 35-K118050-0022-344-C02C-8409
GenBank Accession LN501994 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K118050-0022-344-C02C-8409
ATACATTTTAGAAAGAATAATCATGTTTTTTTAGTCCTCGAAATCTAATTTAACACTGTT
AACTTTTAGTTAGTGTGGCCATGCTAAGATAATTTAGCTAGAAAAGTAATAATTTTGTGT
TTTGTGGGCCAGAAAATAAGTAAAATAATGATTAGAATCCAATTTGTTGTAAAGGCAAGG
CGGCGATCAAATACAGTCACTCTCATTAAAACGCAGAGTTTTTTAAAAAACCCAAATCAC
AGTCGAATTTTCCCGCGCAATAAACCCTAATTTGGATTTGGTGCAGGTTCCAATTGACCA
GCAAATCGGAGCAATTGATTGGCTTCAAGCCCAGAATGAGATTCAGCCTCGCTGTTTCTT
CTCTCGTCGCAGTGACGTTGGTCGTCCCGATCTTCTTCTCGATCTAGCTAACGAGAACGG
AAACGGAAACGGAAACGGAACAGTGTCATCTGATCGTAATCTGGTTAGCGTTGCTGGTAT
CGGCTCTGCAGTTTTCTTCCGTGACCTTGATCC

Last Updated on 06.12.2018 16:11