Genotyping and primer details


Genotyping and primer details for 344C02 - At1g74710
Line ID 344C02
Locus At1g74710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28071260 of line 344C02 Chr1:28071260
   
Insertion allele amplicon 8474 - 13ve
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 13ve: 5'-GTCATGAGAGAAAGGATCAAGGTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28071785
   
wt amplicon (over InsPos) 78wa - 13ve
wt amplicon start Chr1:28070620
wt amplicon end Chr1:28071785
wt amplicon size 1166 bp
Locus specific primer 13ve: 5'-GTCATGAGAGAAAGGATCAAGGTC-3'
2nd locus specific primer 78wa: 5'-ATGTCTATGAATGGTTGTGATGGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11