Sequences from confirmation amplicon


Confirmation sequences for line 353B11 and hit At1g18400 are shown.
Line ID 353B11
Locus At1g18400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6332460
Primers used for PCR BE76 - 8474, show primer details
   
Sequence Name 07-K029122-022-353-B11C-8409
GenBank Accession LN489137 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K029122-022-353-B11C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATGGCTTCATGCATGTTAAAAATA
CTTTTGGATACATATGTTCTTGTTTAACTAAAAGTATCACTTTTATCATAAAATGCTTAA
ATTTGATTCAAGTATAATTAGTGACAATTAATGCCGATGATGACCTTTTCATATGCTATT
TATTGTTTAATGGCCATTAGTTTCTATTTTGATTACAAATATATTAAAAAAGAAGTAATG
AAAAGTTGAAAATGTTAAACTATTATATTTATCTTTGCATTATTACCTTATAACATCCGG
GCACCATATCTTGCAAGCATCTTAATCTCTCGTTTATTTTCCCTCGCCGAACCTGAAATT
TTGATAT
   
Sequence Name 08-K029122-022-353-B11C-BE76
GenBank Accession LN489602 [GenBank]
Sequencing primer gene specific primer, BE76: 5'-CTATATCAAAATTTCAGGTTCGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K029122-022-353-B11C-BE76
GGAAAATAAACGAGAGATTAAGATGCTTGCAAGATATGGTGCCCGGATGTTATAAGGTAA
TAATGCAAAGATAAATATAATAGTTTAACATTTTCAACTTTTCATTACTTCTTTTTTAAT
ATATTTGTAATCAAAATAGAAACTAATGGCCATTAAACAATAAATAGCATATGAAAAGGT
CATCATCGGCATTAATTGTCACTAATTATACTTGAATCAAATTTAAGCATTTTATGATAA
AAGTGATACTTTTAGTTAAACAAGAACATATGTATCCAAAAGTATTTTTAACATGCATGA
AGCCATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACG
ATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATT
TCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11