Genotyping and primer details


Genotyping and primer details for 353B11 - At1g18400
Line ID 353B11
Locus At1g18400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6332460 of line 353B11 Chr1:6332460
   
Insertion allele amplicon 8474 - BE76
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BE76: 5'-CTATATCAAAATTTCAGGTTCGGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6332134
   
wt amplicon (over InsPos) 46dy - BE76
wt amplicon start Chr1:6332134
wt amplicon end Chr1:6332889
wt amplicon size 756 bp
Locus specific primer BE76: 5'-CTATATCAAAATTTCAGGTTCGGC-3'
2nd locus specific primer 46dy: 5'-TTTCGAATATCTAAGATTTGCCGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11