Sequences from confirmation amplicon


Confirmation sequences for line 363H05 and hit At3g43210 are shown.
Line ID 363H05
Locus At3g43210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:15193073
Primers used for PCR 21ie - 8474, show primer details
   
Sequence Name 38-K112562-0022-363-H05C-21ie
GenBank Accession LN503035 [GenBank]
Sequencing primer gene specific primer, 21ie: 5'-TTTAGCAAAGCCTTAGAACCATGT-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K112562-0022-363-H05C-21ie
TAATGAGACATATGATATCATCATTTTATTTCCTGTTATGTGATTTTCCATTTATTGTTA
CAGATCAAAGGCAAGTAGGTGAAACTGCTTTAAACGACAAAAGTTCAAGATCGCATCAGA
TTATTCGGCTGGTATGATCTATACTTATCTGATATGCTGCATGATTGAATTAATATTCTA
TTAAACATGGAGAAGATAGTTTTGCCTAGTTATTTGCTACCATTAGACAGTAAAAGTAAC
CACTTCCCATAATTTCTCATGTTCTCTGCTTTTAATATTCAGACCATCCATAGCAGTCTC
AGGGAAATAGCAGGCTGTGTGCAGTCTTTCATGGCAACTCTGGTACGTCATTTATTATCA
TTGGGTTGAAGCTTCACTTGGTATTGACGACATTTTAATTGGTGCAGAATCTTGTTGATC
TTGCTGGAAGCGAACGTGCTTTTCAAACAAACGCAGATGGTCTAAGATTGAAGGAAGGAA
GCCACATTAACCGTAGTTTATTGACACTTACTACAGTGATCAGAAAACTGAGGAACATGG
ATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCA
   
Sequence Name 14-K112561-0022-363-H05C-8409
GenBank Accession LN492575 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K112561-0022-363-H05C-8409
TTGAATATATCCATGTTCCTCAGTTTTCTGATCACTGTAGTAAGTGTCAATAAACTACGG
TTAATGTGGCTTCCTTCCTTCAATCTTAGACCATCTGCGTTTGTTTGAAAAGCACGTTCG
CTTCCAGCAAGATCAACAAGATTCTGCACCAATTAAAATGTCGTCAATACCAAGTGAAGC
TTCAACCCAATGATAATAAATGACGTACCAGAGTTGCCATGAAAGACTGCACACAGCCTG
CTATTTCCCTGAGACTGCTATGGATGGTCTGAATATTAAAAGCAGAGAACATGAGAAATT
ATGGGAAGTGGTTACTTTTACTGTCTAATGGTAGCAAATAACTAGGCAAAACTATCTTCT
CCATGTTTAATAGAATATTAATTCAATCATGCAGCATATCAGATAAGTATAGATCATACC
AGCCGAATAATCTGATGCGATCTTGAACTTTTGTCGTTTAAAGCAGTTTCACCTACTTGC
CTTTGATCTGTAACAATAAATGGAAAATCACATAACAGGAAATAAAATGATGATATCATA
TGTCTCATTAGTGATTAGTGAAATAATTAGTCATATTTTATATATGTCATCTACATGGTT
CT

Last Updated on 06.12.2018 16:11