Genotyping and primer details


Genotyping and primer details for 363H05 - At3g43210
Line ID 363H05
Locus At3g43210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:15193073 of line 363H05 Chr3:15193073
   
Insertion allele amplicon 8474 - 21ie
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 21ie: 5'-TTTAGCAAAGCCTTAGAACCATGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:15192475
   
wt amplicon (over InsPos) 26ir - 21ie
wt amplicon start Chr3:15192475
wt amplicon end Chr3:15193576
wt amplicon size 1102 bp
Locus specific primer 21ie: 5'-TTTAGCAAAGCCTTAGAACCATGT-3'
2nd locus specific primer 26ir: 5'-TGCTCACTATAGGATGATGATTGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11