Sequences from confirmation amplicon


Confirmation sequences for line 374D04 and hit At5g45710 are shown.
Line ID 374D04
Locus At5g45710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18541298
Primers used for PCR 18hz - 8474, show primer details
   
Sequence Name 30-K112810-0022-374-D04C-18hz
GenBank Accession LN499793 [GenBank]
Sequencing primer gene specific primer, 18hz: 5'-ATTCTTGACGATGAAGCTTTTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K112810-0022-374-D04C-18hz
AGAGATCATCACCATTTCATATGTTTTAGTAAGGAAAGGTGGAAGTGAGCTTGAACCTCC
ATTATTTTCATCCATACCTTAACCACTTCGAATCCAAGACGTTTAAAACCAAACCCGAAC
AAAAAGTCCCTTGATTAAGTCTTATAGTCTGTTGGATAGAACAAGAAGCTGAAATCAAAC
TCAACTAGAGAGACTCATTGAAAACATAAACATTTAATTGCAACAATTCCAGTTTAAGAC
ACAACTGTCAAGTGTTTGAATCAATATACGAATCGATCTCTCAAGCTTTAAGAAGCAAAG
TAATAAATGACAATAAAAACCAAGACTTTTCCCTCACAAGCTTCGAAATTTATTGAAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 05-K112809-0022-374-D04C-8409
GenBank Accession LN488431 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K112809-0022-374-D04C-8409
ATTTTCATAAATTTCGAAGCTTGTGAGGGAAAAGTCTTGGTTTTTATTGTCATTTATTAC
TTTGCTTCTTAAAGCTTGAGAGATCGATTCGTATATTGATTCAAACACTTGACAGTTGTG
TCTTAAACTGGAATTGTTGCAATTAAATGTTTATGTTTTCAATGAGTCTCTCTAGTTGAG
TTTGATTTCAGCTTCTTGTTCTATCCAACAGACTATAAGACTTAATCAAGGGACTTTTTG
TTCGGGTTTGGTTTTAAACGTCTTGGATTCGAAGTGGTTAAGGTATGGATGAAAATAATG
GAGGTTCAAGCTCACTTCCACCTTTCCTTACTAAAACATATGAAATGGTTGATGATTCTT
CTTCTGACTCGGTCGTTGCTTGGAGCGAAAACAACAAAAGCTTCTCGNTCAAGAA

Last Updated on 06.12.2018 16:11