Genotyping and primer details


Genotyping and primer details for 374D04 - At5g45710
Line ID 374D04
Locus At5g45710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:18541298 of line 374D04 Chr5:18541298
   
Insertion allele amplicon 8474 - 18hz
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 18hz: 5'-ATTCTTGACGATGAAGCTTTTGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:18541707
   
wt amplicon (over InsPos) 04mj - 18hz
wt amplicon start Chr5:18540913
wt amplicon end Chr5:18541707
wt amplicon size 795 bp
Locus specific primer 18hz: 5'-ATTCTTGACGATGAAGCTTTTGTT-3'
2nd locus specific primer 04mj: 5'-AAACCTTTCCTTCAATCCTCAAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11