Sequences from confirmation amplicon


Confirmation sequences for line 377E12 and hit At3g29035 are shown.
Line ID 377E12
Locus At3g29035 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:11035162
Primers used for PCR NS35 - 8474, show primer details
   
Sequence Name 96-K102437-0022-377-E12C-8409
GenBank Accession LN515386 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>96-K102437-0022-377-E12C-8409
ATTTTTTTTACAAAATGTTATAAGAATCTATTAGTGTCATCTTTGTATATCCTAGAATCT
AAAGCTAACACATAACGCATATATACACTGGTTTTTAAAAACCCTATATACCCCCTAGTT
AACATCCAAATTTTCGATTTTCCAAATTCAGAATTTCCAAACGCAATCAAGATTCACTGG
ACCAGTGGAACCATAAGAATTGTGACCTTGCCAAGAACTCGCTGTTAGTTCACTCTCTGA
TGAACCTGAATTGTGAAGATTCTTCTTAAATTGGGTTTCTTCATTGTCAAGCAACATCTT
CAGTACTGAAGAATTGTTTTGTAGAAACAGAGGATCGAAGTCTAGTAGAGAACCTATGTT
TGGTAAAAAGTTAGTCGATCCGGAACCAAACATGGTAGTTTTAAAATCGGCCACAAGACT
CTTGTCATCGGTTGTTTGGTCGGAGAAGTAGGTAACGTGAGACAACAACCCACCAAGGGT
GGTCCCGGCTAAAGAGTCTTGTCCTCTACTCTTTAGATATGGAGAAGAATCCATCAGCGG
AGGTAAACCAACGGACATATGCTCCTTCGTACCATCAGTCCGTGTATGAAAA
   
Sequence Name 96-K102438-0022-377-E12C-NS35
GenBank Accession LN515387 [GenBank]
Sequencing primer gene specific primer, NS35: 5'-TTTTTCATACACGGACTGATGGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>96-K102438-0022-377-E12C-NS35
CGTGGTTACTCCGCTGATGGATTCTTCTCCTATCTAAGAGTAGAGGACAAGACTCTTTAG
CCGGGACCACCCTTGGTGGGTTGTTGTCTCACGTTACCTACTTCTCCGACCAAACAACCG
ATGACAAGAGTCTTGTGGCCGATTTTAAAACTACCATGTTTGGTTCCGGATCGACTAACT
TTTTACCAAACATAGGTTCTCTACTAGACTTCGATCCTCTGTTTCTACAAAACAATTCTT
CAGTACTGAAGATGTTGCTTGACAATGAAGAAACCCAATTTAAGAAGAATCTTCACAATT
CAGGTTCATCAGAGAGTGAACTAACAGCGAGTTCTTGGCAAGGTCACAATTCTTATGGTT
CCACTGGTCCAGTGAATCTTGATTGCGTTTGGAAATTCTGAATTTGGAAAATCGAAAATT
TGGATGTTAACTAGGGGGTATATAGGGTTTTTAAAAACAGTGTATATATGCGTTATGTGT
TAGCTTTAGATTCTAGGATATACAAAGATGACACTAATAGATTCTTATAACATTTTGTAA
AAAAAAATCATTGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCG
TTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATA
GGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT
CACGTCCCAAATG
Primers used for PCR NS36 - 8474, show primer details
   
Sequence Name 67-K102506-0022-377-E12C-8409
GenBank Accession LN511783 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K102506-0022-377-E12C-8409
CCATTTACAATTGAAACTCTCTTGCCATTTGAAATCCATTGTGTTTGCATATTTAAGGTG
TTCATAAATTGTCATGCTGAATTGTGTTTGCATATTCTCATTATTTCCCTGTTTGTAATT
AATGATTGTAACATACCTAAGTAGATAATATTAGATTAAAAATATGACTAGTTTACCAAA
TTTTACTTGTTACATTTTGGGTTCTTAATCAAAATCAAAAGGCTGCAAATGGTTGTCTCA
AGTAGAAAATATTAAGTTGAAAAAAACTGAGATCTTAATAAAACTTCTGATAAACAGAAT
AAAAACTAAACAAGTGAAACAATAAGGCTTCATGATGAAAAGTGAATTTCAGGAGATTAC
TGGCAGCAGAGACACGAAAAAAAAACCGAAACTGATTCGGGTCGGGTCATAAGCTGCAAA
GCGGATCCCTAATTTTGATATATGCACATAAAATTATTAGACAGATTCCCCAATTGTTTT
TACAGGGATGTCCCAGTACTTGTGCAATCATAAACAGCCTTTGATACTCACAAAAAAAAA
AACCGAAAATACCGAAAAAAATAAAACCGGCGAAAAAACATAAGTGTGTATTGTCTTTTA
AAAACTATCGGTGTGATAAACAACCTTATATATAAACTAGTAAAACCCTAACCAGGTGTT
TGTTG
   
Sequence Name 67-K102507-0022-377-E12C-NS36
GenBank Accession LN511784 [GenBank]
Sequencing primer gene specific primer, NS36: 5'-AAAGCCCAAAATATCAACAAACAC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K102507-0022-377-E12C-NS36
ACCGATAGTTTCTAAAAGACAATACACACTTATGTTCTTCCGCCGTTTCTATCTTCTTCG
TTATTTTCGTTTTTTTTTTTTGGGAGTATCAAAGGCTGTTTATGATTGCACAAGTACGGG
GACATCCCTGTAAAAACAATTGGGGAATCTGTCTAAAAATTTTATGTGCATATATCAAAA
TTAGGGATCCCCTTTGCACCTTATGACCCGACCCAAATCATTTTCGGTTTTTTTTTCGGG
TCCCTGCGGCCAGAAATCCCCGGAAATTCACTTTTCACCAGGAACCCTTATGGTTTCACT
TGTTAAGTTTTTATTCGGTTTACCAAAATTTTTATTAAAATCCCATTTTTTTTCACCTTA
AAATTTTCTACTTGAAACAACCTTTTGCACCCTTTTGATTTTGATTAAAAACCCAAAATG
TAACAAGTAAAATTTGGAAAACAAGCCAAATTTTTAACCAAAAATTACCTACTTAGGTAT
GTTACAATCTTTATTTACAAACGGGGAAATAATGAAAATTTGCAAACCCATTTCACCAGG
ACATTTTATGAACCCCTTAAATATGCAAACACAAGGGATTTCAAAGGGCAAAAAAGTATT
CATTTGAAAAGGGCTTCTTGTCCGGGAAATCTACTTGGATCACCAATGATTATAATGGTC
AATATGGAAAAAAAAAAAAAGTAATTCCCAATTTTTTTTCATTTCAAAAATGTAAATGTC
CCC

Last Updated on 06.12.2018 16:11