Sequences from confirmation amplicon


Confirmation sequences for line 386G09 and hit At3g57130 are shown.
Line ID 386G09
Locus At3g57130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21149417
Primers used for PCR MH33 - 8474, show primer details
   
Sequence Name 47-K023495-022-386-G09C-8409
GenBank Accession LN506065 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K023495-022-386-G09C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATTCACAAGCTCGACATCAGAG
GAGTCCAATGCACGTCTCATCCTCCTGATCTTCTGGTCTTCGAGGTCTAAAGTCGAGGTT
AGGTCATGGTGGTGAGGCATTAGTGAACGGAGAGGCATTGAAGATTTGAGACGGAGCTCC
TCAATCTTGGCTACAAGTTCGATTGGGAGATGTTTGGCTAGAATCTCTTGTGGAAGACCG
GATTTGGCGATTAAGTAAGAGCAAGTAGTCCATAATTGATGCATGTCTTGCTTTCTTGAA
GCTATGAGCACTTTCATCACATCTTCAATGGAGGCTTTCTCCACCATACTTGTCAAATGT
TTCTGTGAGGGAAAAAAACACAGTTGGCTTTAAAGACAAAACAATATTGAAGTTAGAGAC
ACTATATATCCACCACTTCCATTTCAT
   
Sequence Name 48-K023495-022-386-G09C-MH33
GenBank Accession LN506386 [GenBank]
Sequencing primer gene specific primer, MH33: 5'-TATTCATCCAATGAAATGGAAGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K023495-022-386-G09C-mh33
GATATATAGTGTCTCTAACTTCAATATTGTTTTGTCTTTAAAGCCAACTGTGTTTTTTTC
CCTCACAGAAACATTTGACAAGTATGGTGGAGAAAGCCTCCATTGAAGATGTGATGAAAG
TGCTCATAGCTTCAAGAAAGCAAGACATGCATCAATTATGGACTACTTGCTCTTACTTAA
TCGCCAAATCCGGTCTTCCACAAGAGATTCTAGCCAAACATCTCCCAATCGAACTTGTAG
CCAAGATTGAGGAGCTCCGTCTCAAATCTTCAATGCCTCTCCGTTCACTAATGCCTCACC
ACCATGACCTAACCTCGACTTTAGACCTCGAAGACCAGAAGATCAGGAGGATGAGACGTG
CATTGGACTCCTCTGATGTCGAGCTTGTGAATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAA
GTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAAC
AAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11