Genotyping and primer details


Genotyping and primer details for 386G09 - At3g57130
Line ID 386G09
Locus At3g57130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:21149417 of line 386G09 Chr3:21149417
   
Insertion allele amplicon 8474 - MH33
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MH33: 5'-TATTCATCCAATGAAATGGAAGTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:21149000
   
wt amplicon (over InsPos) 06sq - MH33
wt amplicon start Chr3:21149000
wt amplicon end Chr3:21149917
wt amplicon size 918 bp
Locus specific primer MH33: 5'-TATTCATCCAATGAAATGGAAGTG-3'
2nd locus specific primer 06sq: 5'-TAGCACCAAGATTCAGATAAACCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11