Sequences from confirmation amplicon


Confirmation sequences for line 388C11 and hit At1g06220 are shown.
Line ID 388C11
Locus At1g06220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1902070
Primers used for PCR 30sj - 8474, show primer details
   
Sequence Name 09-K116292-0022-388-C11C-30sj
GenBank Accession LN490347 [GenBank]
Sequencing primer gene specific primer, 30sj: 5'-ACATATCTGCTGCTTCAACAACTG-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K116292-0022-388-C11C-30sj
ATGTATGTTTTGCCAGTGGTACAGCCGGATGGTCCTTCACATTACAATCTTTTGCTAAAA
TGTATGCCAAGCTTCATGGTGTGGCCATGGACGTGGATAAGTTTGCATCTCGACTTTGGG
GAGATGTATATTATCACTCTGATACTAGGGTATTCAAGAGAAGTCCTCCAGTAGGTGGTG
GAGAAAGAGCATTTGTTCAGTTTATTCTGGAGCCCCTATACAAGATATACAGCCAAGTGA
TAGGTGAACATAAGAAGAGTGTAGAGACCACCCTTGCAGAATTAGGAGTGACTCTGAGTA
ATAGTGCATATAAGCTGAACGTCAGACCTCTACTTAGGTTAGCCTGTAGTTCAGTGTTTG
GTTCGGCATCAGGGTTCACTGATATGTTGGTAAAGCCATTTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATTAT
   
Sequence Name 01-K116291-0022-388-C11C-8409
GenBank Accession LN486727 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K116291-0022-388-C11C-8409
ATTTACATTGAATGGCTTTACCAACATATCAGTGAACCCTGATGCCGAACCAAACACTGA
ACTACAGGCTAACCTAAGTAGAGGTCTGACGTTCAGCTTATATGCACTATTACTCAGAGT
CACTCCTAATTCTGCAAGGGTGGTCTCTACACTCTTCTTATGTTCACCTATCACTTGGCT
GTATATCTTGTATAGGGGCTCCAGAATAAACTGAACAAATGCTCTTTCTCCACCACCTAC
TGGAGGACTTCTCTTGAATACCCTAGTATCAGAGTGATAATATACATCTCCCCAAAGTCG
AGATGCAAACTTATCCACGTCCATGGCCACACCATGAAGCTTGGCATACATTTTAGCAAA
AGATTGTAATGTGAAGGACCATCCGGCTGTACCACTGGCAAAACATACATTTCCAGCTGC
AGGGTCTATAAGAGGTAAGTCTCCAGCAGTTGTTGAAGCAGCAGATATGTA

Last Updated on 06.12.2018 16:11