Genotyping and primer details


Genotyping and primer details for 388C11 - At1g06220
Line ID 388C11
Locus At1g06220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:1902070 of line 388C11 Chr1:1902070
   
Insertion allele amplicon 8474 - 30sj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 30sj: 5'-ACATATCTGCTGCTTCAACAACTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1901609
   
wt amplicon (over InsPos) 02tf - 30sj
wt amplicon start Chr1:1901609
wt amplicon end Chr1:1902466
wt amplicon size 858 bp
Locus specific primer 30sj: 5'-ACATATCTGCTGCTTCAACAACTG-3'
2nd locus specific primer 02tf: 5'-CATAGCTTGCATTACACAGAGTCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11