Sequences from confirmation amplicon


Confirmation sequences for line 396G06 and hit At5g46920 are shown.
Line ID 396G06
Locus At5g46920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19055633
Primers used for PCR 18ry - 8474, show primer details
   
Sequence Name 30-K116132-0022-396-G06C-18ry
GenBank Accession LN499864 [GenBank]
Sequencing primer gene specific primer, 18ry: 5'-GAGTTGTTTATCCGACTCTGAGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K116132-0022-396-G06C-18ry
GCATTCAAACAGTTTTGAAAGCTGTTCATTTCATAAAAAGGAGATCGTGATCCGGATCCA
CAGCCTTGTTTTAGAAAGTTTCACGCAACTCAAGCTCATACGAATAATCAGAATGAATAA
GTTTTTGACTACGAATTGCTGAGTGGTATCGGTTTGCAGATAACAGAAAGAAAATTTTGA
AATTTTCTGTTCTTATATTATCCGTGGTTCACTTGCTAAGCTTTATGCTGCAAAGTATAA
ACTTAGATCAAGAGCAAAAGTGTATAAGTTCGCTAATCGGAATCTGAGCCTTCCGTTGTT
GCAGAAGAAAGGTCAGTCACCAGAATATCAGAATCTCTTAAGAATGGGTCTTGCTGAATC
GGTTGATGGACTTGTTTATACAAGAATGTCTCTAGTTCCAGAAACTGATTACTCGCCTTT
CCCGGGTAATTGGAGGCCCGAGCACGAGAAGTTTCTGATCGAGTACTTAACACTCGATGA
ACCGATGTTAGAGAAAAAGAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTA
   
Sequence Name 05-K116131-0022-396-G06C-8409
GenBank Accession LN488496 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K116131-0022-396-G06C-8409
CATCGGTTCATCGAGTGTTAAGTACTCGATCAGAAACTTCTCGTGCTCGGGCCTCCAATT
ACCCGGGAAAGGCGAGTAATCAGTTTCTGGAACTAGAGACATTCTTGTATAAACAAGTCC
ATCAACCGATTCAGCAAGACCCATTCTTAAGAGATTCTGATATTCTGGTGACTGACCTTT
CTTCTGCAACAACGGAAGGCTCAGATTCCGATTAGCGAACTTATACACTTTTGCTCTTGA
TCTAAGTTTATACTTTGCAGCATAAAGCTTAGCAAGTGAACCACGGATAATATAAGAACA
GAAATTCACAATTTTCTTTCTGTTATCTGCAAACCGATACCACTCAGCAATCGTAGTCAA
AAACTTATTCATCTGATTATTCGTATGAGCTTGAGTTGCGTGGAACATTCTAAAACAAGG
CTGTGGATCCGGATCACGATCTCCTTTTATAAATAACAGCTTTCGAAACTGTTTGATGCA
TTGCTTCAAACTCGCTGTAACGGATAAAAGCGTTCCCACGCCTTTTTCGCTTATAATCTT
CCCACCGGTCGCAGTATACCTCAGAG

Last Updated on 06.12.2018 16:11