Genotyping and primer details


Genotyping and primer details for 396G06 - At5g46920
Line ID 396G06
Locus At5g46920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:19055633 of line 396G06 Chr5:19055633
   
Insertion allele amplicon 8474 - 18ry
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 18ry: 5'-GAGTTGTTTATCCGACTCTGAGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:19055055
   
wt amplicon (over InsPos) 41si - 18ry
wt amplicon start Chr5:19055055
wt amplicon end Chr5:19056128
wt amplicon size 1074 bp
Locus specific primer 18ry: 5'-GAGTTGTTTATCCGACTCTGAGGT-3'
2nd locus specific primer 41si: 5'-ACGCGGAACCTGAATAGTTTATTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11