SimpleSearch - Line and FST details
Line specific information
| Line ID | 396G06 |
| Vector Used | pAC161 |
| Line Availability | available as T3 set from NASC (N437998) |
| Segregation Analysis | 50:50:48 |
| Confirmed for Hit | At5g46920 |
| Parent of DUPLO pair | 11873 |
| Parent of pair(s) | none |
| Note |
Gene hit F15L12
| Sequence (A. th genome BLAST matches underlined) | >47-K018299-022-396-G06-8409 GGACATGAGCCATTTACATTGAATATATATAGCAACTTTATCCGCCTCCCTACCCGACTA TTAATCTGTTGCCGGGAAGCTATGGGATCCTCCCTATATGTGAGAGAACTTAGTAACAGG AATTGAGTGCCTTCACTTCAAAAAATGGGAGATCATAAGTGAATGGGCTTGGACGCTTGG TAAATAAAATGAATAAGCTAACTTAGGGCCCATTTATGCTATGGGATCCTCCCTATAGTG AGGAAANGGGGGGCTGNNGCGAATTTATATGTATCAAAAATAAGAA |
| GenBank Accession | BX287084 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr5:24248424 - go to primer design |
| BLAST e Value | 1e-31 |
| Hit Clone Code (BAC ID) | F15L12 |
| Insertion Classification | Genome Hit |
| Confirmation Status | unknown |
Gene hit At5g08520
| Sequence (A. th genome BLAST matches underlined) | >47-K017796-022-396-G06-8409 TATCATGAGATCTCCGGACATGAGCCTTTACATTGAATATATCCTGTTCATCATAGAGTC AGCGCCCTCTTCTTCTTCTCACTGAATCTTCTCCCTCTTGCAGATTTAACACGTATGAGA TCCTCCCTATAAAGAGAGGGATCCTCCCTATAGTGAGAAATCTCGACTGATCTCTTGAGA ACTCGGTCACATCTTGCCTATTCTCAGCTCCCTTCGATAAATGGGATACTCCCTATAGTG AGAGCCTGGTTATACGACAAACCATGCTCATACTCTGATTCATCAGCGATACGATATTGT TCATAATTCGAGAGCGTGAATAACATTCAACCTATGGGATCCTCTCTATAGTGAGACTTC ATCGCCAGCATGGCCATTCCATCCTTCGGGACACCCATAGGGAGGAAGAGGCACACTTCC TGATTCAATCCTATGGGATCCTCATTATGGAGAGAGATCGAGTAAACTGCAACCTCCTTT GTAGCATCCTATGGGATCCTCCCTATAGTGAG |
| GenBank Accession | BX287082 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr5:2757182 - go to primer design |
| BLAST e Value | 2e-21 |
| Hit Clone Code (BAC ID) | F8L15 |
| Hit Gene Code | At5g08520 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | Duplicated homeodomain-like superfamily protein |
| Insertion Classification | CDSi |
| Confirmation Status | failed |
Gene hit At5g46920
| Sequence (A. th genome BLAST matches underlined) | >47-K018295-022-396-G06-8409 GATCATGTGATTTCCCGGACATGAAGCCATTTCTTTTTCTCTAACATCGGTTCATCGAGT GTTAAGTACTCGATCAGAAACTTCTCGTGCTCGGGCCTCCAATTACCCGGGAAAGGCGAG TAATCAGTTTCTGGAACTATGGGATCCTCCCTATAGTGAGAGGCTTCTGTATTCTCCTAT TAGAGAAAAATTGTTCCTTATTCTTCTTGTATCTCCCTTTTGGCGAGCATACTCATTGCT GTTTCATGTAAATTTCTCGGTACATGCCAGCCCTTAATTTTTCTCTATATGCGTGTTCCT AATAATTAACTTCTATTATATGAGAGTTTTCGAGGTTGGACCTACGGTTTTTCGAGAATT GGTTTTATCCAGTTCATGAATAAATCCTCGCCTTTCGATGATTAGTATGGGATCCTCGCT ATAGTGAGAGAGACAAGACTCATTGGGCAATTACACTTAAGCAATCGGGGAGTGAAGGTG TAATTAGCGAAAGGAAAAAAGTGAAGATCTCGGGTAAAGGCGGGGGTGAATTATGCTGTA CCAGAGGATTGGGGGGGCGATACGTAAAGTGAGAACGGAGATAGTGGAGAAAGATAAATA TGCGTGGAGGACTCTTTTTTCTTGGTCCAAAAATATGGAAAACAGGCAGACTCTGAGAGT GGTGGGCCCGAAGATGAAACAAAGGAATAAAAGGAGGAATGGAGGATACGTGACTGGGGA CGAGGTTAAAAGAAAAAAAGAG |
| GenBank Accession | BX287083 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr5:19055633 - go to primer design |
| BLAST e Value | 2e-45 |
| Hit Clone Code (BAC ID) | MQD22 |
| Hit Gene Code | At5g46920 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | Intron maturase, type II family protein |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
|
Last Updated on 10.06.2021 13:37 |
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