Sequences from confirmation amplicon


Confirmation sequences for line 405H12 and hit At4g21090 are shown.
Line ID 405H12
Locus At4g21090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11257910
Primers used for PCR 22qz - 8474, show primer details
   
Sequence Name 24-K115806-0022-405-H12C-22qz
GenBank Accession LN497132 [GenBank]
Sequencing primer gene specific primer, 22qz: 5'-CATGTATCTTACATTCAGGCGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K115806-0022-405-H12C-22qz
GAGTTGATGTTTTCTGAGATAAATTTGAACCTACTTTTCTGTTTTTTCGCCACCTTTCTC
AGAGGTAGTGCTGAAAGATGTACAAAACTTATGATTATTCGAGAATAATGCTTCCTTAAA
ACTCCTAGTTTGCCTTTGCAGCATTGGCTGTCGAGATGAAAGAGCAAAGATATTAAACTA
AAATCAAATATGTAAGAGAACAGCGCAAGTCACCAAAAACCCAAGCTTCATAGCTAAGTC
ACTTCTAAGCAACAGACTCAGGCATTTGCTAGCACCTTGAGCCAAAGCAAAACCCTTTAA
TTCTCTCTATTGGTTGCTGAACATATCTTATCATTAAGCATCACATATCTATAACTGGCT
AAACCATTTCATGATACGGACAAAGATGGTGTATATCTTACAAGCTCCTCAGATTCCTAA
CAAAACCAAACTCAGTATCAACAAGCTTTTAGCCTTTTACCTAATCTACAATTCAGTCGG
AGCCGACCAATTAAGAATTAACAACTCATATCTACAATTGTAAATGGCTTCATGTCTTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGATGTCCG
CA
   
Sequence Name 03-K115805-0022-405-H12C-8409
GenBank Accession LN487626 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K115805-0022-405-H12C-8409
TTTCATTGAAGACTGAAGCCATTTACAATTGTAGATATGAGTTGTTAATTCTTAATTGGT
CGGCTCCGACTGAATTGTAGATTAGGTAAAAGGCTAAAAGCTTGTTGATACTGAGTTTGG
TTTTGTTAGGAATCTGAGGAGCTTGTAAGATATACACCATCTTTGTCCGTATCATGAAAT
GGTTTAGCCAGTTATAGATATGTGATGCTTAATGATAAGATATGTTCAGCAACCAATAGA
GAGAATTAAAGGGTTTTGCTTTGGCTCAAGGTGCTAGCAAATGCCTGAGTCTGTTGCTTA
GAAGTGACTTAGCTATGAAGCTTGGGTTTTTGGTGACTTGCGCTGTTCTCTTACATATTT
GATTTTAGTTTAATATCTTTGCTCTTTCATCTCGACAGCCAATGCTGCAAAGGCAAACTA
GGAGTTTTAAGGAAGCATTATTCTCGAATAATCATAAGTTTTGTACATCTTTCAGCACTA
CCTCTGAGAAAGGTGGCGAAAAAACAGAAAAGTAGGTTCAAATTTATCTCAGAAAACATT
CAACTCTTGACAACAACGAANAATGAGACAACAGCACTCTCGCCTGAATGTANAATACAT
G

Last Updated on 06.12.2018 16:11