Genotyping and primer details


Genotyping and primer details for 405H12 - At4g21090
Line ID 405H12
Locus At4g21090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:11257910 of line 405H12 Chr4:11257910
   
Insertion allele amplicon 8474 - 22qz
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 22qz: 5'-CATGTATCTTACATTCAGGCGAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:11257342
   
wt amplicon (over InsPos) 01rj - 22qz
wt amplicon start Chr4:11257342
wt amplicon end Chr4:11258437
wt amplicon size 1096 bp
Locus specific primer 22qz: 5'-CATGTATCTTACATTCAGGCGAGA-3'
2nd locus specific primer 01rj: 5'-TTCTCTGTGTTCATTTTTGATTCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11