Sequences from confirmation amplicon


Confirmation sequences for line 424D03 and hit At3g11410 are shown.
Line ID 424D03
Locus At3g11410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3584998
Primers used for PCR 27aq - 8474, show primer details
   
Sequence Name 65-K106975-0022-424-D03C-8409
GenBank Accession LN511410 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K106975-0022-424-D03C-8409
ATTTACAATTGATCCATTGCTAACTTCTTTATCCATTTTTTGAAAACTCTTAACCATCGT
CTCTGTCCACTCGTCGGAAGCCATAACTTCAACTTCCTTCTTCACTATATCATGCAACCG
TTCTCTGCACTTCTCCGCAACCTAAAATCAACCAAACAAAACCCTAATTAGTAACTAATC
ACAAACAAAAGAAAAAAATATTACTAAACAGAGGAAATTAACGTACATGAGAGCAGCCAT
GGCCGTCAAAGACACCGTAGAAATGATGATTCTCAGAGTTCCGTTGAAGAAACGAAGGAT
GAATCGAGACAGCGTCTTCCATGTCTCGTCTTCTACCACAAACCGACGTCGTACCGATCT
TAGGTACATCGGAAAAGAAACTCTCTGCTTCCGTAACAGAATTTGAATTCGTAACCGCTG
ATCTAGCCTTCTTATTCTCACTCCTCACATTCGAATCCAGATCTAGATTTCCCGGTAACG
TCGAGAGCACTACTGTCTCACGCTTCTGCCGTTTACGGCAGTTTTCAAGCGGAGGAGCAA
CGGCGGAGTCAGCAACAATTTTGATCGACGGAAGTAGATCTAACCTCCGTCTAAGAGAAG
CTCGAGAAGTTGAATCAACCGGTGCCGCCGGTTCAGTCTCTCCAACAACACCGCAACAAA
TCCCAGCCATTTGATCTCTAACAAAACTTCTCCAATTACAGAAGCAAA
   
Sequence Name 81-K106977-0022-424-D03C-27aq
GenBank Accession LN514075 [GenBank]
Sequencing primer gene specific primer, 27aq: 5'-GGTTTGCTCTGTAAATTGGAGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K106977-0022-424-D03C-27aq
GTTGGAGAGACTGAACCGGTAGGCCCGGTTGATTCAACTTCTCGAGCTTCTCTTAGACGG
AGGTTAGATCTACTTCCGTCGATCAAAATTGTTGCTGACTCCGCCGTTGCTCCTCCGCTT
GAAAACTGCCGTAAACGGCAGAAGCGTGAGACAGTAGTGCTCTCGACGTTACCGGGAAAT
CTAGATCTGGATTCGAATGTGAGGAGTGAGAATAAGAAGGCTAGATCAGCGGTTACGAAT
TCAAATTCTGTTACGGAAGCAGAGAGTTTCTTTTCCGATGTACCTAAGATCGGTACGACG
TCGGTTTGTGGTAGAAGACGAGACATGGAAGACGCTGTCTCGATTCATCCTTCGTTTCTT
CAACGGAACTCTGAGAATCATCATTTCTACGGTGTCTTTGACGGCCATGGCTGCTCTCAT
GTACGTTAATTTCCTCTGTTTAGTAATATTTTTTTCTTTTGTTTGTGATTAGTTACTAAT
TAGGGTTTTGTTTGGTTGATTTTAGGTTGCGGAGAAGTGCAGAGAACGGTTGCATGATAT
AGTGAAGAAGGAAGTTGAAGTTATGGCTTCCGACGAGTGGACAGAGACGATGGTTAAGAG
TTTTCAAAAAATGGATAAAGAAGTTAGCAATGGATCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTC

Last Updated on 06.12.2018 16:11