Genotyping and primer details


Genotyping and primer details for 424D03 - At3g11410
Line ID 424D03
Locus At3g11410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:3584998 of line 424D03 Chr3:3584998
   
Insertion allele amplicon 8474 - 27aq
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 27aq: 5'-GGTTTGCTCTGTAAATTGGAGAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3585689
   
wt amplicon (over InsPos) 16bj - 27aq
wt amplicon start Chr3:3584573
wt amplicon end Chr3:3585689
wt amplicon size 1117 bp
Locus specific primer 27aq: 5'-GGTTTGCTCTGTAAATTGGAGAAG-3'
2nd locus specific primer 16bj: 5'-AGAGATTCACTCACCAATTGCTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11