Sequences from confirmation amplicon


Confirmation sequences for line 470F05 and hit At5g43870 are shown.
Line ID 470F05
Locus At5g43870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17634501
Primers used for PCR 20eq - 8474, show primer details
   
Sequence Name 20-K110827-0022-470-F05C-20eq
GenBank Accession LN495259 [GenBank]
Sequencing primer gene specific primer, 20eq: 5'-AACGTTAACCGCAGAACTAACAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K110827-0022-470-F05C-20eq
ATCTCTGCAGCTTCACACACTTCGCCGCCACCAAAGTCGCGGCAGAAGCCACCGCGGAGT
CATTTTTGGCCACTTGCTCGTCAGTTCCAGAACTCGACTGAGAGGCAGTGGCTGCAGCGA
TAGCAGCCACCGCGGCAGCCACTCCAGCTACAGATACAGCCGCGTGAAGCTGTGCATTTT
GTGCACGCGTCTCTTCTCTCTTCTTCTCTCGCCGGTCCTTTAGCCAACGACCAACAGTTT
TAGACCCTCCGGCGGTGCCGGGAATGGCTGATGAACCACGTATGTGGCCGTTAAAGGAAG
GGCTCACACGGTAGAATTGCTGCATGAATATTTATCATACGTTAAAGTCGTATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
GCGTTATT
   
Sequence Name 04-K110826-0022-470-F05C-8409
GenBank Accession LN487971 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K110826-0022-470-F05C-8409
TTGAATATACGACTTTAACGTATGATAAATATTCATGCAGCAATTCTACCGTGTGAGCCC
TTCCTTTAACGGCCACATACGTGGTTCATCAGCCATTCCCGGCACCGCCGGAGGGTCTAA
AACTGTTGGTCGTTGGCTAAAGGACCGGCGAGAGAAGAAGAGAGAAGAGACGCGTGCACA
AAATGCACAGCTTCACGCGGCTGTATCTGTAGCTGGAGTGGCTGCCGCGGTGGCTGCTAT
CGCTGCAGCCACTGCCTCTCAGTCGAGTTCTGGAACTGACGAGCAAGTGGCCAAAAATGA
CTCCGCGGTGGCTTCTGCCGCGACTTTGGTGGCGGCGAAGTGTGTGGAAGCTGCAGAGAT
TATGGGAGCTGATCGTGAGCACTTGGCCTCCGTTGTTAGTTCTGCGGTTAACGT

Last Updated on 06.12.2018 16:11