Sequences from confirmation amplicon


Confirmation sequences for line 472C02 and hit At1g69640 are shown.
Line ID 472C02
Locus At1g69640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26195693
Primers used for PCR HM95 - 8474, show primer details
   
Sequence Name 15-K101324-0022-472-C02C-8409
GenBank Accession LN492904 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K101324-0022-472-C02C-8409
AACGCAAGTATCGTCCTTTGTTTTGTTTCTTTCTTCATCGTTGATTTGTCAACTCAACAA
CGCCCAAACCCTAAAACAATCCCCAATCATCGTTTCAATAATCAAATTTCCCCAATTGAA
GAATAAAGATCAAAACTTTGGAAGTTTTCCTGTGAAAATAATCTATCCTTTTTGCATCAT
TTGCGTTTTGGTTGATGAAGAGGATCTGAAGAAGCTGAGATTTAGGAATGATGATGGGTT
TTGCTGTATCGGATGAATTGTTGGGTACTGTTGCTCCGATTGTGGTGTACTGGTTGTATT
CTGGGATTTATGTAGCGCTCTCCTCTTTAGAGAGTTATAGATTGCATTCAAAGGTTGAAG
AAGAAGAGAAGAATCTTGTATCGAAATCTTCTGTTGTGAAAGGTGTTCTTGTTCAACAGG
TTGTTCAAGCTGTTGTCGCTATCCTTTTGTTCACGGTACCGTTTTTCTCTTTTCCCATTT
TTGAGTTGTTTTGCTTTTGTGAAATTGAAGTGAAAAGTTAGGTAAAACATATTGGGGAAT
TTTGATATCTGACGCTGACTTTTTATGTGTGGAATTGGATGGTTAAGATTTTTGTTTTTA
AAAAGGTTAACTTTTTGGGTTGATGTTGTCTTTCGACCCATAAAGAGAAATGGGTTATGT
ATTAATGATCTTAAAGTTGGATCATATATTGACTGTTCTAACAATAGTTAAGAGCTTTAG
AGGAAAGTATCAATAAGGATTGTTCTGTGATTTGGCAAT
   
Sequence Name 15-K101341-0022-472-C02C-HM95
GenBank Accession LN492905 [GenBank]
Sequencing primer gene specific primer, HM95: 5'-GGAGCCTAAGATAGAGTCCTTGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K101341-0022-472-C02C-HM95
TCTGCCAGTCAAACCCTATTGCCAATCACAGAACAATCCCTATTGCTATTTTCCTCTAAA
GCTCTTAACTATTGTTAGAACAGTCAATATATGATCCAACTTTAAGATCATTAATACATA
ACCCATTTCTCTTTATGGGTCGAAAGACAACATCAACCCAAAAAGTTAACCTTTTTAAAA
ACAAAAATCTTAACCATCCAATTCCACACATAAAAAGTCAGCGTCAGATATCAAAATTCC
CCAATATGTTTTACCTAACTTTTCACTTCAATTTCACAAAAGCAAAACAACTCANAAATG
GGAAAAGAGAAAAACGGTACCGTGAACAAAAGGATAGCGACAACAGCTTGAACAACCTGT
TGAACAAGAACACCTTTCACAACAGAAGATTTCGATACAAGATTCTTCTCTTCTTCTTCA
ACCTTTGAATGCAATCTATAACTCTCTAAAGAGGAGAGCGCTACATAAATCCCAGAATAC
AACCAGTACACCACAATCGGAGCAACAGTACCCAACAATTCATCCGATACAGCAAAACCC
ATCATCATTCCTAAATCTCAGCTTCTTCAGATCCTCTTCATCAACCAAAACGCAAATGAT
GCANAAAGGATAGATTATTTTCACAGGAAAACTTCCCAAGTTTTGATCTTTATTCTTCAA
TTGGGGAAATTTGATTATTGAAACGATAATNGGGGATTGTTTTANGGTTTGGGCGTTGTT
GAGTTGACNAATCAACGATGAAAAAAGAAA

Last Updated on 06.12.2018 16:11