Genotyping and primer details


Genotyping and primer details for 472C02 - At1g69640
Line ID 472C02
Locus At1g69640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:26195693 of line 472C02 Chr1:26195693
   
Insertion allele amplicon 8474 - HM95
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer HM95: 5'-GGAGCCTAAGATAGAGTCCTTGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:26194867
   
wt amplicon (over InsPos) 09lt - HM95
wt amplicon start Chr1:26194867
wt amplicon end Chr1:26196218
wt amplicon size 1352 bp
Locus specific primer HM95: 5'-GGAGCCTAAGATAGAGTCCTTGGT-3'
2nd locus specific primer 09lt: 5'-CGAAAATGAGTTTCTTGAAAAGGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11