Sequences from confirmation amplicon


Confirmation sequences for line 475D06 and hit At3g59440 are shown.
Line ID 475D06
Locus At3g59440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21970241
Primers used for PCR 32aq - 8474, show primer details
   
Sequence Name 04-K106755-0022-475-D06C-8409
GenBank Accession LN487936 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K106755-0022-475-D06C-8409
TTACAATTGAAATATCCTATAGAGATTAAAAAAAAGCATATCTCTGAATTATTATTATTT
ACGTCCCCATCTCTCTTCCTCTATAAATAGTATAAGCAGAGACAATATTCATTTCAAGTT
CCTCTGTATACCAAACTATCAAAAAAACTCTCTCCATTTCTGTTCTCAACAAAGCCAAGA
GTTATGGTGAGAGTCTTTCTTCTCTACAACCTCTTTAACTCCTTTCTTCTTTGTTTAGTT
CCCAAGAAGCTTAGAGTTTTCTTCCCTCCTTCTTGGTACATCGACGACAAGAACCCACCA
CCGCCTGATGAATCGGAAACTGAATCTCCGGTAGATCTAAAACGAGTGTTTCAGATGTTC
GACAAGAACGGAGATGGACGCATCACAAAGGAAGAGCTGAACATTTCTCTAG
   
Sequence Name 20-K106756-0022-475-D06C-32aq
GenBank Accession LN495230 [GenBank]
Sequencing primer gene specific primer, 32aq: 5'-TCTAGAGAATCGTTCAGCTCTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K106756-0022-475-D06C-32aq
ACATCTGAACACTCGTTTTAGATCTACCGGAGATTCAGTTTCCGATTCATCAGGCGGTGG
TGGGTTCTTGTCGTCGATGTACCAAGAAGGAGGGAAGAAAACTCTAAGCTTCTTGGGAAC
TAAACAAAGAAGAAAGGAGTTAAAGAGGTTGTAGAGAAGAAAGACTCTCACCATAACTCT
TGGCTTTGTTGAGAACAGAAATGGAGAGAGTTTTTTTGATAGTTTGGTATACAGAGGAAC
TTGAAATGAATATTGTCTCTGCTTATACTATTTATAGAGGAAGAGAGATGGGGACGTAAA
TAATAATAATTCAGAGATATGCTTTTTTTTAATCTCTATAGGATATATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
Primers used for PCR 04ax - 8474, show primer details
   
Sequence Name 45-K118837-0022-475-D06C-04ax
GenBank Accession LN505696 [GenBank]
Sequencing primer gene specific primer, 04ax: 5'-AATGATCATACCCTTGAATCTTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K118837-0022-475-D06C-04ax
TCACTTCAGTGATTAATTTCCCAAGTACTTAACGTTACTTTTAGTTTATCCTTTTAACTC
CTTTTTAATTTTCTGAATCAATACCAGGTAAATCAGGGGGGTTCAAACTTAGTCTATCTT
CTTAACTCTTTTTTGTTTTTAAATGTTTTGTAGACTATGTAAACTCTCATCCTAAATCTA
CTTTTTAACTTTTCTATCGATAGCAATAAAAAGTTGTAAAGTATCTACACAAAAACAAGA
TGAAATGGTCAAAAGTCTGTGACTAGCTAGTGTTTGTATTTTAATTCAGAAAGACTTTAA
TGAGTTTATAGTCGTGCTCTTTAGTGTTAAAATAACAATTACTCCTAGACTTTTAGTTAT
TTTTTATTGCATTCTTGGATATCCTCCAACAATATTGTATTATTCAAAAAATTTTTTACT
AAAGTCCATAAAGCAATGAGGTAACGTCTAGGTCAATTAATGGATGGATCATCCAACGGT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATA
   
Sequence Name 21-K118836-0022-475-D06C-8409
GenBank Accession LN495878 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K118836-0022-475-D06C-8409
CATTTCACCGTTGGATGATCCATCCATTAATTGACCTAGACGTTACCTCATTGCTTTATG
GACTTTAGTAAAAAATTTTTTGAATAATACAATATTGTTGGAGGATATCCAAGAATGCAA
TAAAAAATAACTAAAAGTCTAGGAGTAATTGTTATTTTAACACTAAAGAGCACGACTATA
AACTCATTAAAGTCTTTCTGAATTAAAATACAAACACTAGCTAGTCACAGACTTTTGACC
ATTTCATCTTGTTTTTGTGTAGATACTTTACAACTTTTTATTGCTATCGATAGAAAAGTT
AAAAAGTAGATTTAGGATGAGAGTTTACATAGTCTACAAAACATTTAAAAACAAAAAAGA
GTTAAGAAGATAGACTAAGTTTGAACCCCCCTGATTTACCTGGTATTGATTCAGAAAATT
AAAAAGGAGTTAAAAGGATAAACTAAAAGTAACGTTAAGTACTTGGGAAATTTAATCTAC
TGGAAGTTGGAGGATACGCGTGATCAAGCAAGATTCAAGGGTATGATCAT

Last Updated on 06.12.2018 16:11