Genotyping and primer details


Genotyping and primer details for 475D06 - At3g59440
Line ID 475D06
Locus At3g59440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:21970241 of line 475D06 Chr3:21970241
   
Insertion allele amplicon 8474 - 04ax
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 04ax: 5'-AATGATCATACCCTTGAATCTTGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:21969717
   
Insertion allele amplicon 8474 - 32aq
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 32aq: 5'-TCTAGAGAATCGTTCAGCTCTTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:21970652
   
wt amplicon (over InsPos) 04ax - 32aq
wt amplicon start Chr3:21969717
wt amplicon end Chr3:21970652
wt amplicon size 936 bp
Locus specific primer 32aq: 5'-TCTAGAGAATCGTTCAGCTCTTCC-3'
2nd locus specific primer 04ax: 5'-AATGATCATACCCTTGAATCTTGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11