Sequences from confirmation amplicon


Confirmation sequences for line 476D03 and hit At5g53400 are shown.
Line ID 476D03
Locus At5g53400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21663261
Primers used for PCR 68rq - 8474, show primer details
   
Sequence Name 14-K116018-0022-476-D03C-68rq
GenBank Accession LN492655 [GenBank]
Sequencing primer gene specific primer, 68rq: 5'-TAACTCCTGAGTGAACAATGATCG-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K116018-0022-476-D03C-68rq
ATGATACTTTGCAGAGGATCAAAAGGTGATTTCGATTCTCTTGACTAAGTCTGATCAGAT
GGAGTGGTGGAAATGCTGTGTGAAAGGTGAACCTGAAATCGATACTCAGAAAGTTGAACC
AGAGACTAGTAAATTAGGTGACCTTGATCCAGAAACTCGCTCGACTGTTGAGAAAATGAT
GGTATGTCTTCTAAAGAATTGTTTCTTTCTTTATTCAAGTTTCTCGAATTAGAAACTGAA
GTGTGTTTTTCTTTGTTGTCTTTCTATCCATAGTTTGATCAAAGGCAAAAGCAGATGGGT
CTTCCAACAAGCGAGGAGCTACAGAAGCAAGAGATCCTTAAGAAATTCATGTCTGAGGTA
AACAATAAAATTCTCCAATATACAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTC
   
Sequence Name 01-K116017-0022-476-D03C-8409
GenBank Accession LN486717 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K116017-0022-476-D03C-8409
TATATTGGAGAATTTTATTGTTTACCTCAGACATGAATTTCTTAAGGATCTCTTGCTTCT
GTAGCTCCTCGCTTGTTGGAAGACCCATCTGCTTTTGCCTTTGATCAAACTATGGATAGA
AAGACAACAAAGAAAAACACACTTCAGTTTCTAATTCGAGAAACTTGAATAAAGAAAGAA
ACAATTCTTTAGAAGACATACCATCATTTTCTCAACAGTCGAGCGAGTTTCTGGATCAAG
GTCACCTAATTTACTAGTCTCTGGTTCAACTTTCTGAGTATCGATTTCAGGTTCACCTTT
CACACAGCATTTCCACCACTCCATCTGATCAGACTTAGTCAAGAGAATCGAAATCACCTT
TTGATCCTCTGCAAAGTATCATTTTGAGAATTATATAGCAATAAGCACTCTTGTTTACCA
ACAAAGTTCAATATAACGATCATTGT

Last Updated on 06.12.2018 16:11