Genotyping and primer details


Genotyping and primer details for 476D03 - At5g53400
Line ID 476D03
Locus At5g53400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:21663261 of line 476D03 Chr5:21663261
   
Insertion allele amplicon 8474 - 68rq
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 68rq: 5'-TAACTCCTGAGTGAACAATGATCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:21662808
   
wt amplicon (over InsPos) 01rz - 68rq
wt amplicon start Chr5:21662808
wt amplicon end Chr5:21663650
wt amplicon size 843 bp
Locus specific primer 68rq: 5'-TAACTCCTGAGTGAACAATGATCG-3'
2nd locus specific primer 01rz: 5'-AGGACATAATCGAACAGAGGAGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11