Sequences from confirmation amplicon


Confirmation sequences for line 498C10 and hit At5g39930 are shown.
Line ID 498C10
Locus At5g39930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15985253
Primers used for PCR 30fm - 8474, show primer details
   
Sequence Name 65-K111632-0022-498-C10C-30fm
GenBank Accession LN511429 [GenBank]
Sequencing primer gene specific primer, 30fm: 5'-GTGGTGTGTGAAAATAATGAATGC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K111632-0022-498-C10C-30fm
ACGAAAAAAAAATCGGCTCGTACTATTCAACCTCGTAGTCGTACGTATTATATATTGACG
AGAGAAAAATTGTTGGGAGATAAATCCTATATATATATACGGTGTATATATACTGTGTAT
ACATACATATCTTAAAGATTTGTACAAATACATATCTTATTGTTGGAGATACTTATAGAT
TTTATACATACAGTGGATTTTTTAAAAACAAAATCAAGTACATGTACTTATTATAAAGAG
AAAACAAAACAACCAAATTTTTCGTTAAATCTTCAATTCAACAAACAGAGTATATAGGAG
AATAAACTGGAGCATCTTCAAAGAAGTTGAAAAGATATATGTAGTAGAGTTTACAACTTA
AAAATAATAATAAAAAACTCCTTGTTCTATCTTTAGTTTCTTGCTTCTCAAGATATATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATTTCC
GCA
   
Sequence Name 49-K111631-0022-498-C10C-8409
GenBank Accession LN506884 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K111631-0022-498-C10C-8409
TCATTGAATTATCTTGAGAAGCAAGAAACTAAAGATAGAACAAGGAGTTTTTTATTATTA
TTTTTAAGTTGTAAACTCTACTACATATATCTTTTCAACTTCTTTGAAGATGCTCCAGTT
TATTCTCCTATATACTCTGTTTGTTGAATTGAAGATTTAACGAAAAATTTGGTTGTTTTG
TTTTCTCTTTATAATAAGTACATGTACTTGATTTTGTTTTTAAAAAATCCACTGTATGTA
TAAAATCTATAAGTATCTCCAACAATAAGATATGTATTTGTACAAATCTTTAAGATATGT
ATGTATACACAGTATATATACACAGTATATATATATAGGATTTATCTCCCAACAATTTTT
CTCTCGTCAATATATACTACGTACGACTACGAGGTTGAATAGTACGAGCCGATTTTTTTT
TTCGTTCTATCGATGCAACGCCTACGTAAGTTCTTTGTGTAATGCATTCATTATTTTCAC
ACACC

Last Updated on 06.12.2018 16:11