Genotyping and primer details


Genotyping and primer details for 498C10 - At5g39930
Line ID 498C10
Locus At5g39930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:15985253 of line 498C10 Chr5:15985253
   
Insertion allele amplicon 8474 - 30fm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 30fm: 5'-GTGGTGTGTGAAAATAATGAATGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:15984779
   
wt amplicon (over InsPos) 05gs - 30fm
wt amplicon start Chr5:15984779
wt amplicon end Chr5:15985635
wt amplicon size 857 bp
Locus specific primer 30fm: 5'-GTGGTGTGTGAAAATAATGAATGC-3'
2nd locus specific primer 05gs: 5'-GCAAGTATATCGAATCGGTGATTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11