Sequences from confirmation amplicon


Confirmation sequences for line 499C03 and hit At1g72330 are shown.
Line ID 499C03
Locus At1g72330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27235955
Primers used for PCR AM09 - 8474, show primer details
   
Sequence Name 20-K105918-0022-499-C03C-AM09
GenBank Accession LN495214 [GenBank]
Sequencing primer gene specific primer, AM09: 5'-TTGCGTTCTTATTCTGGTTAGGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K105918-0022-499-C03C-AM09
ATTTCTGCAAGCAAGAGGGTTTGGTTCTTTTAGCTGATGAAGTCTACCAAGAAAATGTTT
ACGTCCCTGACAAAAAGTTCCATTCTTTCAAGAAAGTGGCTCGGTCTTTGGGCTATGGAG
AAAAAGATATCTCGTTAGTCTCGTTCCAATCAGTCTCCAAAGGTAAAAGAACAACTTTTA
CTGTCCAATGCTTGTCATCTAAAAAATGGGGTTTTTGTTTTTAATATTCTTCTGTTGATA
GGATATTATGGAGAATGTGGAAAAAGAGGAGGTTACATGGAGGTTACTGGATTCACTTCT
GATGTAAGAGAACAGATATACAAAATGGCTTCTGTGAATCTTTGTTCTAACATCTCTGGT
CAAATTCTTGCTAGCCTTGTCATGAGCCCACCCAAGGTAACACCCTAGTTCTCTCTAATC
GGTTTTCGCTAATATGATCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 20-K105919-0022-499-C03C-8409
GenBank Accession LN495215 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K105919-0022-499-C03C-8409
CGGAATGAGCCATTTACATTGAATATATCCTGGATCATATTAGCGAAAACCGATTAGAGA
GAACTAGGGTGTTACCTTGGGTGGGCTCATGACAAGGCTAGCAAGAATTTGACCAGAGAT
GTTAGAACAAAGATTCACAGAAGCCATTTTGTATATCTGTTCTCTTACATCAGAAGTGAA
TCCAGTAACCTCCATGTAACCTCCTCTTTTTCCACATTCTCCATAATATCCTATCAACAG
AAGAATATTAAAAACAAAAACCCCATTTTTTAGATGACAAGCATTGGACAGTAAAAGTTG
TTCTTTTACCTTTGGAGACTGATTGGAACGAGACTAACGAGATATCTTTTTCTCCATAGC
CCAAAGACCGAGCCACTTTCTTGAAAGAATGGAACTTTTTGTCAGGGACGTAAACATTTT
CTTGGTAGACTTCATCAGCTAAAAGAACCAAACCCTCTTGCTTGCAGAAATTAACAATGT
CACGCTGGTTTTCTTCCGCAAGAACCTAACCAGAAAGNAAACGCA

Last Updated on 06.12.2018 16:11