Genotyping and primer details


Genotyping and primer details for 499C03 - At1g72330
Line ID 499C03
Locus At1g72330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:27235955 of line 499C03 Chr1:27235955
   
Insertion allele amplicon 8474 - AM09
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AM09: 5'-TTGCGTTCTTATTCTGGTTAGGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:27235469
   
wt amplicon (over InsPos) 08ua - AM09
wt amplicon start Chr1:27235469
wt amplicon end Chr1:27236437
wt amplicon size 969 bp
Locus specific primer AM09: 5'-TTGCGTTCTTATTCTGGTTAGGTT-3'
2nd locus specific primer 08ua: 5'-CAAAACCCATTATTCAAGTTCGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11