Sequences from confirmation amplicon


Confirmation sequences for line 500F07 and hit At4g11370 are shown.
Line ID 500F07
Locus At4g11370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:6908083
Primers used for PCR 30ww - CR3S, show primer details
   
Sequence Name 52-K118966-0022-500-F07C-30ww
GenBank Accession LN507974 [GenBank]
Sequencing primer gene specific primer, 30ww: 5'-TATACTTATCTTCAGCTGCCGGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K118966-0022-500-F07C-30ww
ATATCTCACCATTTTTATTATACACACTTTATTCTTGTCTCTATAGTTCCACATCTCAAT
CTAGCTCTCTTCTCTATCTTTCTCTAAATCATGGGTCTTCCTGAAGATTTCATCACCGAG
CTTCAGATTCCAAGTTACATATTAAAGATACTTTACGTCATCGGTTTCTTTAGAGACATC
GTCGATGCTCTTTGTCCTTACATTGGTCTACCTCGTTTTCTAGACCACAACGAGACCTCT
GCACCCGATCTGACCCGACACGCTCTCTCTACGTCAGCGAGTCTTGCTAACGAGTTGATC
CCGGTGGTTCGGTTCTCGGATCTTCCGACCGATCCGGAAGATTGTTGTACGGTTTGTTTG
TCAGATTTTGAGTCCGACGATAAGGTTAGGCAGCTACACCTGGATTTTGGTTTTAGGAAT
TAGAAATTTTATTGATAGAAGTATTTTACAAATACAAATACATACTAAGGGTTTCTAAAA
TGCTCAA
   
Sequence Name 28-K118965-0022-500-F07C-CR3S
GenBank Accession LN499066 [GenBank]
Sequencing primer T-DNA primer, CR3S: 5'-TTGAGCATATAAGAAACCCTTAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K118965-0022-500-F07C-CR3S
TTCTTCATAAATTTCTATTCCTAAACAAAATCCAGGTGTAGCTGCCTAACCTTATCGTCG
GACTCAAAATCTGACAAACAAACCGTACAACAATCTTCCGGATCGGTCGGAAGATCCGAG
AACCGAACCACCGGGATCAACTCGTTAGCAAGACTCGCTGACGTAGAGAGAGCGTGTCGG
GTCAGATCGGGTGCAGAGGTCTCGTTGTGGTCTAGAAAACGAGGTAGACCAATGTAAGGA
CAAAGAGCATCGACGATGTCTCTAAAGAAACCGATGACGTAAAGTATCTTTAATATGTAA
CTTGGAATCTGAAGCTCGGTGATGAAATCTTCAGGAAGACCCATGATTTAGAGAAAGATA
GAGAAGAGAGCTAGATTGAGATGTGGAACTATAGAGACAAGAATAAAGTGTGTATAATAA
AAAATGGTGAGATTTATAATTGTGTTTGGAGTACAATTCAAACCGGCAGCTGAGATTTAA
GTATAATTCNCCCCNTTTTTTATTATACACCACTTTATTCTTGTCTCTATAGTTCCACAT
CTCAATCTAGCTCTCTTCTCTATCTTTCTCTAAATCGTGGGTCTTCCTGAAGATTTCATC
ACCGAGCTTCAGATTCGCAGTTACATATTAAGGATACTTTACGTTCATCGGTTTCTTTAG
AGACATCGTCGATGCTCTTTGTCCTTACATTGGTCTACCTCGTTTT

Last Updated on 06.12.2018 16:11